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快速蛋白萃取試劑組
Rapid Protein Extraction

Minute™ Protein Extraction Kits
The world’s leading spin column based protein extraction kits

就是快速 » 使用創新＂spin column＂技術萃取蛋白質，時間快，純度高

Invent Biotechnologies
Minute™ Total Protein Kit		 Major Brand #1
Total Protein Extraction Kit		 Major Brand #2
Total Protein Extraction Kit		 Major Brand #3
Protein Extraction Reagent
Step 1 Vortex briefly Homogenize tissue for 20 seconds Add reagent to sample Shake for 10 minutes
Step 2 Transfer to spin column Transfer to dry ice Vortexing for 1 minute Centrifuge for 15 minutes
Step 3 Spin for 30 seconds Homogenize tissue for 20 seconds Incubation on ice for 10 minutes Transfer to a new tube
Step 4 - Rotate for 20 minutes Repeat step 2 and 3 for 1hour -
Step 5 - Centrifuge for 20 minutes Centrifige for 30 minutes -
Step 6 - Transfer to a new tube Thansfer to a new tube -
Step 7 - - - -
Total Time 1–5 minute(s) 42 minutes 140 minutes 25 minutes

就是方便 » 僅需四個步驟，操作步驟少=錯誤少、組間差異小、快速

Invent Biotechnologies
Minute™ Total Protein Kit		 Major Brand #1
Total Protein Extraction Kit		 Major Brand #2
Total Protein Extraction Kit		 Major Brand #3
Total Protein Extraction Kit
Step 1 Suspend sample in reagent Suspend sample in reagent Add reagent to sample Suspend sample in reagent
Step 2 Vortex briefly Homogenize tissue Vortexing Place on a shaker
Step 3 Transfer to spin column Transfer to dry ice Put sample on ice and wait Shaking and waiting
Step 4 Spin for 30 seconds Homogenize tissue Vortexing and incubation Waiting......
Step 5
Rotate in a shaker Vortexing and Incubation Still Waiting......
Step 6 Centrifuge Vortexing and Incubation Centrifuge and waiting
Step 7 Transfer to a new tube Vortexing and incubation Waiting......
Step 8 - Votexing and incubation Still Waiting......
Step 9 - Vortexing and Incubation Transfer to a new tube
Step 10 - Centrifuge -
Step 11 - Transfer to a new tube -

微量樣品適用 » 微量樣品的好幫手，高蛋白濃度需求的好工具

Invent 其他廠牌
樣品量 0.3 x 106 cells > 2-5 x 106 cells
萃取體積 20 uL 50-100 uL

2.5x less sample size = 2.5x more concentrated sample

Selection Guide of Minute™ Protein Extraction / Isolation Kits

Cat. No
Starting Materials
Status of Extracted Proteins
Yield
(mg/mL)
Protocol Time
(min)
SD-001 / SD-002 Cultured Animal Cells and Tissues Denatured / Native 2-8 1-8
SC-003 Mammalian Cultured Cells / Tissues Native 1-3 6-8
SN-006 Cultured Animal Cells and Tissues Denatured / Native 1-4 5-8
SM-005 Mammalian Cultured Cells / Tissues Native Detergent / EDTA-Free 1-3 20-45
SM-005-P Plant Cells / Tissues Native Detergent / EDTA-Free 1-2 <60
MP-007 Mammalian Cultured Cells / Tissues Native Detergent / EDTA-Free 1-3 25-30
SD-008 / SN-009 Fresh Plant Tissues Denatured / Native 2-8 5-8
SN-010 Fresh Plant Tissues Native 1-5 6-8
CP-011 Fresh Plant Tissues (5-200 mg / sample) Chloroplast (>90%) Intact 1-4 5
SB-004 Cultured Bacterial Cells Denatured 2-3 2-3
NE-013 Cultured cells or Cells from Tissues Native 1-3 45
HP-014 Cultured cells or Cells from Tissues Denatured 1-2.5 <10
YT-015 Cultured Microbes Denatured / Native 2-5 <10
YD-016 Cultured Microbes Denatured / Native 2-4 <10
YM-017 Cultured Yeast Native 2-3 60
MM-018 Plant tissues Native 2-3 60
PN-019 PAGE / Agarose gels Denatured / Native Variable 10-20
HD-021 Keratinized tissues Denatured 1-2 5-O/N
AT-022 Adipose Tissues / Adipocytes Native / Denatured 2-3 20
AF-023 Adipose Tissues / Adipocytes Native 0.5-1 20
FE-025 FFPE Denatured 2-3 60
MS-026 Mammalian Cultured Cells / Tissues Denatured 2-8 60-O/N
EI-027 Body Fluids Native Variable 30-O/N
ED-028 Mammalian Cultured Cells / Tissues Native 0.02-0.1 120-180
AN-029 White and Brown Adipose Tissues Native 0.5-1 <60
SE-030 Saliva Native Variable 120-O/N

invent Products 2022

Cat. No
Product Name
Storage Temp (°C)
SD-001/SN-002 Minute TM Total Protein Extraction Kit  for Animal Cultured Cells/Tissues (50 Tests) RT
SC-003 Minute TM Cytoplasmic and Nuclear Fractionation kit (50 Tests) 4°C
SN-006 Minute TM Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues (50 Tests) 4°C
SM-005 Minute TM Plasma Membrane Protein Isolation Kit (50 Tests) -20
SM-005-p Minute TM Plasma Membrane Protein Isolation Kit for Plants (50 Tests) -20
MP-007 Minute TM Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests) -20
MM-038 Minute TM Mitochondria Isolation Kit for Muscle Tissues/Cultured Muscle Cells (50 Tests)  -20
SD-008/SN-009 Minute TM Total Protein Extraction Kit for Plant Tissues (50 Tests) RT
SN-010 Minute TM Detergent-Free Plant Protein Extraction Kit (50 Tests) 4°C
CP-011 Minute TM Chloroplast Isolation Kit (50 Tests) -20
SB-004 Minute TM Bacterial Total Protein Extraction Kit (50 Tests) RT
SC-012 Minute TM Single Cell Isolation Kit (50 Tests) 4°C
NE-013 Minute TM Nuclear Envelope Protein Extraction Kit (50 Tests) -20
HP-014 Minute TM Histon/DNA Binding  Protein Extraction Kit (50 Tests) RT
YT-015 Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) RT
YD-016 Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests) RT
YM-017 Minute™ Yeast Mitochondria Enrichment Kit (50 tests) 0
MM-018 Minute™ Plant Microsomal Membrane Extraction Kit (50 Tests) -20
PN-019 Minute™ Protein/Nucleic Acid Extraction Kit from Gels (20 tests) RT
HD-021 Minute™ Protein Extraction Kit for Hair and Nails (20 Tests) RT
AT-022 Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Tests) 4°C
AF-023 Minute™ Adipose Tissue Fractionation Kit (20 Tests) 4°C
NI-024 MinuteTM Detergent-Free Nuclei Isolation Kit (20 Tests) 4°C/-20°C (Buffer B)
BN-020 MinuteTM Single Nucleus Isolation Kit for Neuronal Tissues/Cells (20 preps)  4°C
SN-047 MinuteTM Single Nucleus Isolation Kit for Tissues/Cells (20 preps)  4°C
AN-029 MinuteTM Nuclei and Cytosol Isolation Kit for Adipose Tissues (20 Tests) 4°C
FE-025 MinuteTM Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests) RT
MS-026 MinuteTM Total Protein Extraction Kit for Mass Spectrometry (50 Tests) RT
EI-027 MinuteTM Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG) RT
ED-028 MinuteTM Endosome Isolation and Cell Fractionation Kit (20 Tests) 4°C
SE-030 MinuteTM Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG) RT
CS-031 MinuteTM Cell Suspension Isolation from Fresh/Frozen Tissues (50 tests) 4°C
NT-032 MinuteTM Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 tests)  4°C
NM-033 MinuteTM Detergent-Free Nuclear Matrix Protein Extraction Kit (20 tests)  4°C
LY-034 MinuteTM Lysosome Isolation Kit (20 tests)  4°C
ER-035 MinuteTM High Fidelity ER Isolation Kit for Liver Tissue (20 tests)  4°C
ER-036 MinuteTM ER Enrichment Kit  (20 tests)  4°C
GO-037 MinuteTM Golgi Apparatus Enrichment Kit (20 tests) 4°C
LR-039 MinuteTM Lipid Raft Isolation Kit for Mammalian Cells/Tissues (20 tests)  4°C
LR-042 MinuteTM Lipid Raft Isolation Kit for Mammalian Cells/Tissues (20 tests)  4°C
PT-040 MinuteTM Cytosolic Proteasome Enrichment Kit (20 tests)  4°C
PN-041 MinuteTM Nuclear Proteasome Enrichment Kit (20 tests)  4°C
PF-045 MinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests)  4°C
SV-043 MinuteTM Native Protein Precipitation Kit from Saliva (20 tests)  RT
ML-044 MinuteTM Organic Solvent-Free Milk Lipid Depletion Kit (20 tests) RT
RD-046 MinuteTM Rubisco Depletion Kit (20 tests)  4°C
PR-048 MinuteTM Plant ER Enrichment Kit (20 tests)  4°C
PG-049 MinuteTM Plant Golgi Apparatus Enrichment Kit (20 tests)  4°C
PE-050 MinuteTM Plant Endosome Enrichment Kit (20 tests)  4°C
PL-051 MinuteTM Plant Lipid Raft Isolation Kit (20 tests)  4°C
SY-052 MinuteTM Synaptosome Isolation Kit (50 preps) NEW! 4°C
SA-01-SK MinuteTM Total Protein Extraction Kit for Skin Tissue (50 tests)  RT
SA-02-BT MinuteTM Total Protein Extraction Kit for Bone Tissue (50 tests)  RT
SA-03-BV MinuteTM Total Protein Extraction Kit for Blood Vessels (50 tests)  RT
SA-04-TD MinuteTM Total Protein Extraction Kit for Tendons (50 tests)  RT
SA-05-IM MinuteTM Total Protein Extraction Kit for Intestines and Mesentery (50 tests)  RT
SA-06-MS MinuteTM Total Protein Extraction Kit for Muscles (50 tests)  RT
SA-07-IS MinuteTM Total Protein Extraction Kit for Insects (50 tests)  RT
WA-002 Minute™ Ab Enhancer (30 ml) RT
WA-003 Minute™ WB Stripping Solution (250 ml) RT
WA-004 MinuteTM Ponceau S Stain (250 ml) RT
WA-005 Minute TM Peroxidase Suppressor (30 ml) RT
WA-006 Minute TM High Efficiency Protein Precipitation kit  RT
WA-007 MinuteTM RBC Lysis Buffer (250 ml) RT
WA-008 MinuteTM WB Blocking Solution (250 ml) RT
WA-009 MinuteTM Denaturing Protein Solubilization Reagent (10 ml) RT
WA-010 MinuteTM Non-Denatured Protein Solubilization Reagent (10 ml) RT
WA-011 MinuteTM Protein Solubilization Reagent for MS (2 ml) RT
WA-012 MinuteTM SDS-Remover (10 ml) RT
WA-013 MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml)  RT
WA-014 MinuteTM Anti-Clumping Nuclei Storage Buffer NEW! 4°C

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